Protein purification : principles, high resolution methods, and applications / Jan-Christer Janson and Lars Ryden.
By: Janson, Jan-Christer
.
Contributor(s): Rydén, Lars.
Material type: 
BookPublisher: New York Chichester : Wiley, 1998Edition: 2nd ed.Description: x,664p. : ill. ; 23cm.ISBN: 0471186260 (m); 9780471186267.Subject(s): Chromatographic analysis | Proteins -- Purification | Electrophoresis | Chromatography | Biochemistry | Organic chemistry | ScienceDDC classification: 547.75 JAN Summary: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
| Item type | Current library | Call number | Copy number | Status | Barcode | |
|---|---|---|---|---|---|---|
| General lending | MTU Kerry North Campus Library First Floor Main | 547.75 JAN (Browse shelf(Opens below)) | 1 | Available | 38888000014617 | |
| General lending | MTU Kerry North Campus Library First Floor Main | 547.75 JAN (Browse shelf(Opens below)) | 1 | Available | 38888000014666 |
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Enhanced descriptions from Syndetics:
Praise for the First Edition of Protein Purification:
"Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." -Trends in Analytical Chemistry.
"[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." -FEBS Letters.
This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
* Gel filtration and ion exchange chromatography
* Affinity and covalent chromatography
* Reversed phase and hydrophobic interaction chromatography
* Electrophoresis in gels
* Immunoelectrophoresis and capillary electrophoresis
* Protein mapping by two-dimensional polyacrylamide gel electrophoresis
* Protein elution and blotting methods
* Large-scale electrophoresis processes.
Previous ed.: 1989.
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Table of contents provided by Syndetics
- Preface (p. vii)
- Contributors (p. ix)
- Part I Introduction (p. 1)
- 1 Introduction to Protein Purification (p. 3)
- Part II Chromatography (p. 41)
- 2 Introduction to Chromatography (p. 43)
- 3 Gel Filtration (p. 79)
- 4 Ion Exchange Chromatography (p. 145)
- 5 Chromatofocusing (p. 207)
- 6 High-Resolution Reversed-Phase Chromatography (p. 239)
- 7 Hydrophobic Interaction Chromatography (p. 283)
- 8 Immobilized Metal Ion Affinity Chromatography (p. 311)
- 9 Covalent Chromatography (p. 343)
- 10 Affinity Chromatography (p. 375)
- 11 Affinity Partitioning of Proteins Using Aqueous Two-Phase Systems (p. 443)
- Part III Electrophoresis (p. 461)
- 12 Electrophoresis in Gels (p. 463)
- 13 Isoelectric Focusing (p. 495)
- 14 Immunoelectrophoresis (p. 529)
- 15 Protein Mapping by Two-Dimensional Polyacrylamide Electrophoresis (p. 557)
- 16 Protein Elution and Blotting Techniques (p. 587)
- 17 Capillary Electrophoretic Separations (p. 603)
- 18 Large-Scale Electrophoretic Processes (p. 651)
- Index (p. 679)
Reviews provided by Syndetics
CHOICE Review
Anyone engaging in the purification of proteins would be well served by keeping a copy of Protein Purification on their bookshelves. This book is easy to read, well referenced, and examines the major methods of isolating proteins. The whole process is covered, from the selection of a suitable starting material, followed by the initial fractionation, and ultimately the techniques available and equipment useful in obtaining the desired final product. The theories behind the various types of chromatography and electrophoresis are discussed in some detail as well as their strengths and weaknesses. Tables containing values pertinent to the methods being discussed are thoughtfully provided. Additionally, examples of the use of the techniques using model proteins are given, along with illustrations documenting the progress of the purification. This book will provide the novice with a solid basis for planning a purification scheme and may compel an experienced individual to try unexplored ways to optimize a procedure already being employed. Any laboratory contemplating or practicing protein purification will gladly pay the price for this useful reference. Graduate; faculty; professionals. J. M. Tomich Kansas State UniversityAuthor notes provided by Syndetics
JAN-CHRISTER JANSON is Adjunct Professor of Technical Biochemistry at Uppsala University and Principal Scientist at Amersham Pharmacia Biotech in Sweden.LARS RYDEN is Assistant Professor of Biochemistry at Uppsala University in Sweden.