MTU Library Catalogue

Syndetics cover image
Image from Syndetics

HPLC, a practical user's guide / Marvin C. McMaster.

By: McMaster, Marvin C.
Material type: materialTypeLabelBookPublisher: New York, N.Y. : Wiley-VCH, c1994Description: xii, 211 p. : ill. ; 25 cm.ISBN: 0471185868 (acidfree paper).Subject(s): High performance liquid chromatographyDDC classification: 543.8 MCM
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Kerry North Campus Library First Floor Main 543.8 MCM (Browse shelf(Opens below)) 1 Available 38888000459804
Total holds: 0

Enhanced descriptions from Syndetics:

HPLC: A Practical User's Guide is essential for chromatographers needing a proven training manual for setting up and running an HPLC system, for optimizing and cleaning columns and separations, and for obtaining separations and maximizing information from their system.

Relying upon 15 years of laboratory and field troubleshooting experience, the author explains the basic techniques and shows how to use them to optimize separations. Proven methods are provided for starting up and maintaining HPLC systems, cleaning and troubleshooting column to the system problems, and systematic column treatment and methods development, both manual and automated.

Coverage includes preparative HPLC, Diode Array, UV detectors, recent advances in HPLC/mass spectrometry, and a discussion of hardware configurations and interfacing to computers and data acquisition devices. A guide to separations, a glossary of terms, and a programmed self-learning guide are also included.

Includes bibliographical references (p. 203-204) and index.

Table of contents provided by Syndetics

  • Preface
  • I HPLC Primer
  • 1 Advantages and Disadvantages of HPLC
  • 1.1 How It Works
  • 1.1.1 A Separation Model of the Column
  • 1.1.2 Basic Hardware: A Quick, First Look
  • 1.1.3 Use of Solvent Gradients
  • 1.1.4 Ranges of Compounds
  • 1.2 Other Ways to Make My Separation
  • 1.2.1 FPLC-Fast Protein Liquid Chromatography
  • 1.2.2 LC-Traditional Liquid Chromatography
  • 1.2.3 GLC-Gas Liquid Chromatography
  • 1.2.4 SFC-Supercritical Fluid Chromatography
  • 1.2.5 TLC-Thin Layer Chromatography
  • 1.2.6 EP-Electrophoresis
  • 1.2.7 CZE-Capillary Zone Electrophoresis
  • 2 Selecting an HPLC System
  • 2.1 Characteristic Systems
  • 2.1.1 Finding a Fit: Detectors and Data Processing
  • 2.1.2 System Models: Gradient Versus Isocratic
  • 2.1.3 Vendor Selection
  • 2.1.4 Brand Names and Clones
  • 2.1.5 Hardware-Service-Support
  • 2.2 System Cost Estimates
  • 2.2.1 Type I System-QC Isocratic (Cost: $10-15,000)
  • 2.2.2 Type II System-Research Gradient (Cost: $20-25,000)
  • 2.2.3 Type III System-Automated Clinical (Cost: $25-35,000)
  • 2.2.4 Type IV System-Automated Methods (Cost: $30-50,000)
  • 2.3 Columns
  • 2.3.1 Sizes: Analytical and Preparative
  • 2.3.2 Separating Modes: Selecting Only What You Need
  • 2.3.3 Tips on Column Use
  • 3 Running Your Chromatograph
  • 3.1 Set-up and Start-up
  • 3.1.1 Hardware Plumbing 101: Tubing and Fittings
  • 3.1.2 Connecting Components
  • 3.1.3 Solvent Clean-up
  • 3.1.4 Water Purity Test
  • 3.1.5 Start-up System Flushing
  • 3.1.6 Column Preparation and Equilibration
  • 3.2 Sample Preparation and Column Calibration
  • 3.2.1 Sample Clean-up
  • 3.2.2 Plate Counts
  • 3.3 Your First Chromatogram
  • 3.3.1 Reproducible Injection Techniques
  • 3.3.2 Simple Scouting for a Mobile Phase
  • 3.3.3 Examining the Chromatogram
  • 3.3.4 Basic Calculations of Results
  • II HPLC Optimization
  • 4 Separation Models
  • 4.1 Partition
  • 4.1.1 Separation Parameters
  • 4.1.2 Efficiency Factor
  • 4.1.3 Separation (Chemistry) Factor
  • 4.2 Ion Exchange Chromatography
  • 4.3 Size Exclusion Chromatography
  • 4.4 Affinity Chromatography
  • 5 Column Preparation
  • 5.1 Column Variations
  • 5.2 Packing Materials and Hardware
  • 5.3 Column Selection
  • 6 Column Aging, Diagnosis, and Healing
  • 6.1 Packing Degrading-Bonded-Phase Loss
  • 6.2 Dissolved Packing Material-End Voids
  • 6.3 Bound Material
  • 6.4 Pressure Increases
  • 6.5 Column Channeling-Center-Voids
  • 6.6 Normal Phase, Ion Exchange, and Size Columns
  • 6.7 Zirconium and Polymer Columns
  • 7 Partition Chromatography Modifications
  • 7.1 Reverse-Phase and Hybrid Silica
  • 7.1.1 Ionization Suppression
  • 7.1.2 Ion Pairing
  • 7.1.3 Organic Modifiers
  • 7.1.4 Chelation
  • 7.2 Acidic Phase Silica
  • 7.3 Reverse-Phase Zirconium
  • 7.4 Partition Mode Selection
  • 8 "Nonpartition" Chromatography
  • 8.1 Ion Exchange
  • 8.1.1 Cationic:Weak and Strong
  • 8.1.2 Anionic:Weak and Strong
  • 8.2 Size Exclusion
  • 8.2.1 Organic Soluble Samples
  • 8.2.2 Hydrophilic Protein Separation
  • 8.3 Affinity Chromatography
  • 8.3.1 Column Packing Modification
  • 8.3.2 Chelation and Optically Active Columns
  • 9 Hardware Specifics
  • 9.1 System Protection
  • 9.1.1 Filters, Guard Columns, and Saturation Columns
  • 9.1.2 Inert Surfaces and Connections
  • 9.2 Pumping
  • 9.2.1 High- and Low-Pressure Mixing Controllers
  • 9.2.2 Checking Gradient Performance
  • 9.3 Injectors and Autosamplers
  • 9.4 Detectors
  • 9.4.1 Mass Dependent Detecto