MTU Library Catalogue

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An introduction to immunocytochemistry / Julia M. Polak and Susan Van Noorden.

By: Polak, Julia M.
Contributor(s): Van Noorden, Susan | Royal Microscopical Society (Great Britain).
Material type: materialTypeLabelBookSeries: Microscopy handbooks ; 11.Publisher: Oxford : Oxford University Press, 1987Edition: Rev.ed.Description: 60 pages.Content type: text Media type: unmediated Carrier type: volumeISBN: 0198564155 .Subject(s): ImmunocytochemistryDDC classification: 571.964
Contents:
Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Immunofluorescence methods -- Immunoenzyme methods -- Modifications of the basic methods -- Specificity problems and essential controls -- In vitro methods for testing antibodies and checking antigens -- Applications of immunocytochemistry -- Microscopy.
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Bishopstown Library Lending 571.964 (Browse shelf(Opens below)) 1 Available 00043459
General lending MTU Bishopstown Library Lending 571.964 (Browse shelf(Opens below)) 1 Available 00043460
General lending MTU Bishopstown Library Lending 571.964 (Browse shelf(Opens below)) 1 Available 00043776
Total holds: 0

Enhanced descriptions from Syndetics:

This work contains a concise exposition of the different immunocytochemical methods for identifying tissue constituents, discussing ways of preparing tissues according to the antigen to be localized and the most commonly used methods. The text is illustrated with explanatory diagrams and photomicrographs. An appendix contains step-by-step instructions for many of the basic techniques at light and electron microscopical levels. Other sections deal with sources of error and nonspecificity, and techniques for recording results photographically. For this revised edition, the authors have updated the text in light of the latest developments and added 20 new references to the literature.

Includes bibliographical references (pages 52-58) and index.

Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Immunofluorescence methods -- Immunoenzyme methods -- Modifications of the basic methods -- Specificity problems and essential controls -- In vitro methods for testing antibodies and checking antigens -- Applications of immunocytochemistry -- Microscopy.

Table of contents provided by Syndetics

  • 1 Introduction
  • 2 Production of Antibodies
  • 3 Requirements for Immunocytochemistry
  • 4 Immunofluorescence Methods
  • 6 Modifications of the Basic Methods
  • 7 Specificity Problems and Essential Controls
  • 8 In Vitro Methods for Testing Antibodies and Checking Antigens
  • 9 Applications of Immunocytochemistry
  • 10 Microscopy
  • 11 Appendix: Technical Notes
  • A.1 Immunostaining by Indirect Method
  • A.2 Peroxidase Antiperoxidase Method
  • A.3 Cryostat Sections
  • A.4 Intensification of the Peroxidase Reaction
  • A.5 Protease Digestion
  • A.6 To Prevent Sections Becoming Detached from Slides
  • A.7 Peroxidase Development by Alternatives to DAB Methods
  • A.8 Notes
  • A.9 Development of Alkaline Phosphabase to Give a Blue End-Product
  • A.10 Post-Embedding Electron Microscopical Immunocytochemistry Using an Indirect Immunogold Method