Enhanced descriptions from Syndetics:

KEY BENEFIT The latest edition of this successful text provides readers with a modern and complete experience in experimental biochemistry. Part I, Theory and Experimental Techniques, provides in-depth theoretical discussion organized around important techniques. A valuable reference for instructors and students, it's particularly useful to instructors who prefer to use their own customized experiments. Part II, Experiments, offers optimum flexibility through 15 tested experiments designed to accommodate the capabilities of laboratories and students at most four-year schools. Alternate methods are suggested and labs may be divided into manageable hour segments. The book offers the latest safety and environmental precautions in each experiment to inform students and instructors of potential hazards and proper disposal of materials. For anyone interested in science.

Table of contents provided by Syndetics

• Part 1 Theory and Experimental Techniques
• Chapter 1 Introduction to the Biochemistry Laboratory (p. 3)
• A. Safety in the Laboratory (p. 4)
• B. The Laboratory Notebook and Experiment Reports (p. 8)
• Details of Experimental Write-up (p. 9)
• C. Cleaning Laboratory Glassware (p. 10)
• Glassware (p. 10)
• Quartz and Glass Cuvettes (p. 11)
• D. Preparation and Storage of Solutions (p. 11)
• Water Quality (p. 11)
• Solution Preparation (p. 11)
• E. Quantitative Transfer of Liquids (p. 13)
• Filling a Pipet (p. 13)
• Disposable Pasteur Pipets (p. 14)
• Calibrated Pipets (p. 14)
• Automatic Pipetting Systems (p. 16)
• Cleaning and Drying Pipets (p. 18)
• F. Statistical Analysis of Experimental Data (p. 18)
• Analysis of Experimental Data (p. 19)
• Determination of the Mean, Sample Deviation, and Standard Deviation (p. 20)
• Statistical Analysis in Practice (p. 23)
• Study Problems (p. 25)
• Further Reading (p. 27)
• On the Web (p. 28)
• Chapter 2 General Laboratory Procedures (p. 29)
• A. pH, Buffers, Electrodes, and Biosensors (p. 29)
• Measurement of pH (p. 30)
• Biochemical Buffers (p. 32)
• Selection of a Biochemical Buffer (p. 34)
• The Oxygen Electrode (p. 37)
• Ion-Selective Electrodes and Biosensors (p. 39)
• B. Measurement of Protein Solutions (p. 41)
• The Biuret and Lowry Assays (p. 41)
• The Bradford Assay (p. 43)
• The BCA Assay (p. 44)
• The Spectrophotometric Assay (p. 44)
• C. Measurement of Nucleic Acid Solutions (p. 46)
• The Spectrophotometric Assay (p. 46)
• Other Assays for Nucleic Acids (p. 46)
• D. Techniques for Sample Preparation (p. 48)
• Dialysis (p. 48)
• Ultrafiltration (p. 49)
• Lyophilization and Centrifugal Vacuum Concentration (p. 52)
• Study Problems (p. 55)
• Further Reading (p. 56)
• On the Web (p. 57)
• Chapter 3 Purification and Identification of Biomolecules by Chromatography (p. 59)
• A. Introduction to Chromatography (p. 59)
• Partition versus Adsorption Chromatography (p. 60)
• B. Planar Chromatography (Paper and Thin-Layer) (p. 61)
• Preparation of the Stationary Support (p. 62)
• Solvent Development of the Support (p. 63)
• Detection and Measurement of Components (p. 64)
• Applications of Planar Chromatography (p. 64)
• C. Gas Chromatography (GC) (p. 65)
• Instrumentation (p. 65)
• Selection of Operating Conditions (p. 68)
• Analysis of GC Data (p. 68)
• Advantages and Limitations of GC (p. 69)
• D. Column Chromatography (p. 70)
• Operation of a Chromatographic Column (p. 71)
• Packing the Column (p. 71)
• Loading the Column (p. 72)
• Eluting the Column (p. 72)
• Collecting the Eluent (p. 73)
• Detection of Eluting Components (p. 73)
• E. Ion-Exchange Chromatography (p. 74)
• Ion-Exchange Resins (p. 75)
• Selection of the Ion Exchanger (p. 76)
• Choice of Buffer (p. 78)
• Preparation of the Ion Exchanger (p. 78)
• Using the Ion-Exchange Resin (p. 78)
• Storage of Resins (p. 79)
• F. Gel Exclusion Chromatography (p. 79)
• Theory of Gel Filtration (p. 79)
• Physical Characterization of Gel Chromatography (p. 80)
• Chemical Properties of Gels (p. 81)
• Selecting a Gel (p. 83)
• Gel Preparation and Storage (p. 83)
• Operation of a Gel Column (p. 84)
• Applications of Gel Exclusion Chromatography (p. 85)
• G. High-Performance Liquid Chromatography (HPLC) (p. 87)
• Instrumentation (p. 89)
• Stationary Phases in HPLC (p. 92)
• The Mobile Phase (p. 95)
• Solvents for HPLC Operation (p. 96)
• Gradient Elution in HPLC (p. 97)
• Sample Preparation and Selection of HPLC Operating Conditions (p. 98)
• FPLC--A Modification of HPLC (p. 98)
• H. Affinity Chromatography and Immunoadsorption (p. 99)
• Chromatographic Media (p. 100)
• The Immobilized Ligand (p. 101)
• Attachment of Ligand to Matrix (p. 101)
• Experimental Procedure for Affinity Chromatography (p. 104)
• I. Perfusion Chromatography (p. 106)
• Study Problems (p. 107)
• Further Reading (p. 108)
• Chromatography on the Web (p. 109)
• Chapter 4 Characterization of Proteins and Nucleic Acids by Electrophoresis (p. 111)
• A. Theory of Electrophoresis (p. 111)
• B. Methods of Electrophoresis (p. 113)
• Polyacrylamide Gel Electrophoresis (PAGE) (p. 113)
• Nucleic Acid Sequencing Gels (p. 121)
• Agarose Gel Electrophoresis (p. 122)
• Pulsed Field Gel Electrophoresis (PFGE) (p. 126)
• Isoelectric Focusing of Proteins (p. 127)
• Two-Dimensional Electrophoresis of Proteins (p. 130)
• Capillary Electrophoresis (CE) (p. 130)
• Immunoelectrophoresis (IE) (p. 132)
• C. Practical Aspects of Electrophoresis (p. 133)
• Instrumentation (p. 133)
• Reagents (p. 133)
• Staining and Detecting Electrophoresis Bands (p. 134)
• Protein and Nucleic Acid Blotting (p. 136)
• Analysis of Electrophoresis Results (p. 137)
• Study Problems (p. 138)
• Further Reading (p. 139)
• Electrophoresis on the Web (p. 140)
• Chapter 5 Spectroscopic Analysis of Biomolecules (p. 141)
• A. Ultraviolet-Visible Absorption Spectrophotometry (p. 142)
• Principles (p. 142)
• Instrumentation (p. 146)
• Applications (p. 150)
• B. Fluorescence Spectrophotometry (p. 157)
• Principles (p. 157)
• Instrumentation (p. 160)
• Applications (p. 161)
• Difficulties in Fluorescence Measurements (p. 162)
• C. Other Spectroscopic Methods (p. 163)
• Nuclear Magnetic Resonance Spectroscopy (p. 163)
• Mass Spectrometry (p. 167)
• Study Problems (p. 168)
• Further Reading (p. 169)
• Spectroscopy on the Web (p. 170)
• Chapter 6 Radioisotopes in Biochemical Research (p. 171)
• A. Origin and Properties of Radioactivity (p. 171)
• Introduction (p. 171)
• Isotopes in Biochemistry (p. 174)
• Units of Radioactivity (p. 175)
• B. Detection and Measurement of Radioactivity (p. 176)
• Liquid Scintillation Counting (p. 176)
• Geiger-Muller Counting of Radioactivity (p. 181)
• Scintillation Counting of [gamma] Rays (p. 182)
• Background Radiation (p. 183)
• Applications of Radioisotopes (p. 183)
• Statistical Analysis of Radioactivity Measurements (p. 184)
• C. Radioisotopes and Safety (p. 184)
• Preparation for the Experiment (p. 185)
• Performing the Experiment (p. 186)
• Study Problems (p. 187)
• Further Reading (p. 187)
• Radioisotopes on the Web (p. 188)
• Chapter 7 Centrifugation in Biochemical Research (p. 189)
• A. Basic Principles of Centrifugation (p. 189)
• B. Instrumentation for Centrifugation (p. 193)
• Low-Speed Centrifuges (p. 193)
• High-Speed Centrifuges (p. 195)
• Ultracentrifuges (p. 198)
• C. Applications of Centrifugation (p. 201)
• Preparative Techniques (p. 201)
• Analytical Measurements (p. 202)
• Care of Centrifuges and Rotors (p. 206)
• Study Problems (p. 207)
• Further Reading (p. 208)
• Centrifugation on the Web (p. 208)
• Part 2 Experiments
• Experiment 1 Using the Computer in Biochemical Research (p. 211)
• Experiment 2 Structural Analysis of a Dipeptide (p. 227)
• Experiment 3 Using Gel Filtration to Study Ligand-Protein Interactions (p. 243)
• Experiment 4 Isolation and Characterization of Bovine Milk [alpha]-Lactalbumin (p. 257)
• Experiment 5 Kinetic Analysis of Tyrosinase (p. 279)
• Experiment 6 Purification and Characterization of Triacylglycerols in Natural Oils (p. 303)
• Experiment 7 Identification of Serum Glycoproteins by SDS-PAGE and Western Blotting (p. 321)
• Experiment 8 Isolation and Characterization of Plant Pigments (p. 333)
• Experiment 9 Photoinduced Proton Transport through Chloroplast Membranes (p. 345)
• Experiment 10 Isolation, Subfractionation, and Enzymatic Analysis of Beef Heart Mitochondria (p. 357)
• Experiment 11 Measurement of Cholesterol and Vitamin C in Biological Samples (p. 371)
• Experiment 12 Activity and Thermal Stability of Gel-immobilized Peroxidase (p. 389)
• Experiment 13 Extraction and Characterization of Bacterial DNA (p. 399)
• Experiment 14 Plasmid DNA Isolation and Characterization by Electrophoresis (p. 415)
• Experiment 15 The Action of Restriction Endonucleases on Plasmid or Viral DNA (p. 431)
• Appendices
• Appendix I Properties of Common Acids and Bases (p. 443)
• Appendix II Properties of Common Buffer Compounds (p. 444)
• Appendix III pK[subscript a] Values and pH[subscript I] Values of Amino Acids (p. 445)
• Appendix IV Molecular Weights of Some Common Proteins (p. 446)
• Appendix V Common Abbreviations Used in This Text (p. 447)
• Appendix VI Units of Measurement (p. 449)
• Appendix VII Table of the Elements (p. 451)
• Appendix VIII Values of t for Analysis of Statistical Confidence Limits (p. 453)
• Appendix IX Answers to Selected Study Problems (p. 454)
• Index (p. 467)