MTU Library Catalogue

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DNA cloning. 2, expression systems : a practical approach / edited by D.M. Glover and B.D. Hames.

Contributor(s): Glover, David M | Hames, B. D.
Material type: materialTypeLabelBookSeries: Practical approach series ; v. 149.Publisher: Oxford ; New York : IRL Press, 1994-Edition: 2nd ed.Description: v. : illustrations ; 23 cm.Content type: text Media type: unmediated Carrier type: volumeISBN: 0199634785.Subject(s): Molecular cloning -- Laboratory manuals | Recombinant DNA -- Laboratory manualsDDC classification: 572.8633
Contents:
Screening expression libraries with antibody and protein probes / Benjamin Margolis and Richard A. Young -- Expression of foreign proteins in E. coli using plasmid vectors and purification of specific polyclonal antibodies / Jim A. Williams, James A. Langeland, Bruce S. Thalley, James B. Skeath and Sean B. Carroll -- Purification of over-produced proteins from E. coli cells / Reinhard Grisshammer and Kiyoshi Nagai -- Production of monoclonal antibodies against proteins expressed in E. coli / Steven M. Picksley and David P. Lane -- Expression of cloned genes in yeast / Michael A. Romanos, Carol A. Scorer and Jeffrey J. Clare -- Interaction trap cloning with yeast / Russell L. Finley Jr and Roger Brent -- The baculovirus expression system / Gunvanti Patel and Nicholas C. Jones.
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Bishopstown Library Lending 572.8633 (Browse shelf(Opens below)) 1 Available 00013854
Total holds: 0

Enhanced descriptions from Syndetics:

Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories.

Includes bibliographical references and index.

Screening expression libraries with antibody and protein probes / Benjamin Margolis and Richard A. Young -- Expression of foreign proteins in E. coli using plasmid vectors and purification of specific polyclonal antibodies / Jim A. Williams, James A. Langeland, Bruce S. Thalley, James B. Skeath and Sean B. Carroll -- Purification of over-produced proteins from E. coli cells / Reinhard Grisshammer and Kiyoshi Nagai -- Production of monoclonal antibodies against proteins expressed in E. coli / Steven M. Picksley and David P. Lane -- Expression of cloned genes in yeast / Michael A. Romanos, Carol A. Scorer and Jeffrey J. Clare -- Interaction trap cloning with yeast / Russell L. Finley Jr and Roger Brent -- The baculovirus expression system / Gunvanti Patel and Nicholas C. Jones.

Table of contents provided by Syndetics

  • 1 Screening expression libraries with antibody and protein probes
  • 2 Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
  • 3 Purification of over-produced proteins from E.coli cells
  • 4 Production of monoclonal antibodies against proteins expressed in E.coli
  • 5 Expression of cloned genes in yeast
  • 6 Intraction trap cloning with yeast
  • 7 The baculovirus expression system

Author notes provided by Syndetics

B. D. Hames is at University of Leeds. D. Glover is at University of Dundee.