MTU Library Catalogue

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Immunological reagents and solutions: a laboratory handbook / Bassam B. Damaj.

By: Damaj, Bassam B, 1968-.
Material type: materialTypeLabelBookPublisher: Natick, MA : Eaton, 2000Description: xii, 142 p. ; 23 cm. + spiral.ISBN: 1881299295.Subject(s): Experimental immunology -- Laboratory manuals | Biological reagents -- Laboratory manualsDDC classification: 571.96072
Contents:
Introduction -- ELISA -- Antibodies -- Western blots -- Electrophoresis -- Cell culture -- Stains -- Calcium and intracellular pH fluorescent measurement -- Immunoprecipitations -- Immunostaining -- PCR -- General.
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Bishopstown Library Lending 571.96072 (Browse shelf(Opens below)) 1 Available 00082904
General lending MTU Bishopstown Library Lending 571.96072 (Browse shelf(Opens below)) 1 Available 00082903
Total holds: 0

Enhanced descriptions from Syndetics:

This handbook provides detailed instruction for the preparation of cellular and molecular reagents commonly used in immunological protocols and employed in other fields of biological research. It is designed for students, researchers, and laboratory technicians working in immunology or in another field using immunological techniques and reagents. Topics include ELISA, antibodies, western blots, electrophoresis, cell culture, stains, calcium and intracellular pH florescent measurement, immunoprecipitations, immunostaining, and PCR. Damaj received his PhD in microbiology and immunology from Laval University. He now works in research and development for a private corporation. Comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

"A BioTechniques Books Publication.".

Bibliography: (pages 117-122) and index.

Introduction -- ELISA -- Antibodies -- Western blots -- Electrophoresis -- Cell culture -- Stains -- Calcium and intracellular pH fluorescent measurement -- Immunoprecipitations -- Immunostaining -- PCR -- General.

Table of contents provided by Syndetics

  • Introduction (p. 1)
  • Section 1. Elisa (p. 3)
  • Section 2. Antibodies (p. 7)
  • A. Labeling (p. 10)
  • B. Buffers (p. 12)
  • Section 3. Western Blots (p. 19)
  • Section 4. Electrophoresis (p. 25)
  • Section 5. Cell Culture (p. 33)
  • A. Sera (p. 33)
  • B. Media (p. 35)
  • Section 6. Stains (p. 39)
  • Section 7. Calcium and Intracellular pH Fluorescent Measurement (p. 45)
  • A. Buffers (p. 45)
  • B. Dye (p. 48)
  • C. Ionophores (p. 48)
  • Section 8. Immunoprecipitations (p. 49)
  • A. Buffers (p. 50)
  • B. Detergents (p. 53)
  • Section 9. Immunostaining (p. 55)
  • A. Medium (p. 55)
  • B. Solutions (p. 56)
  • Section 10. PCR (p. 57)
  • A. Buffers (p. 58)
  • B. Gels (p. 64)
  • C. Solutions (p. 66)
  • Section 11. General (p. 75)
  • References (p. 117)
  • Commercial Sources (p. 123)
  • Quick Reference Tables (p. 129)
  • About the Author (p. 133)
  • Index (p. 135)

Author notes provided by Syndetics

Bassam B. Damaj, PhD, is a group leader of chemokines in the Research and Development section of Tanabe Research Laboratories USA (TRL)-Tanabe Seiyaku Co., in San Diego, CA