MTU Library Catalogue

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Immunoassays : a practical approach / edited by James P. Gosling.

Contributor(s): Gosling, James P.
Material type: materialTypeLabelBookSeries: Practical approach series: 228.Publisher: Oxford ; New York : Oxford University Press, 2000Description: xix, 304 p. : ill. ; 26 cm.ISBN: 0199637113 ; 0199637105.Subject(s): Immunoassay -- Laboratory manualsDDC classification: 616.0756
Contents:
Analysis by specific binding / James P. Gosling -- Raising antibodies / Marian M. Kane and John N. Banks -- Choosing and characterizing antibodies / Anthony Forde and John Coley -- Labels and endpoints / Wajdi Abdul-Ahad and Martin Brett -- Solid-phase reagents / Robert S. Matson -- Standards and immunogens / M. Quasim Chaudry -- Optimization / Eric Ezan and Jacques Grassi -- Validation / Peter O\'Fegan -- Data processing / Barry Nix and David Wild -- Quality assurance / John Seth.
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Bishopstown Library Lending 616.0756 (Browse shelf(Opens below)) 1 Available 00115988
General lending MTU Bishopstown Library Lending 616.0756 (Browse shelf(Opens below)) 1 Available 00079269
Total holds: 0

Enhanced descriptions from Syndetics:

Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.

Includes bibliographical references and index.

Analysis by specific binding / James P. Gosling -- Raising antibodies / Marian M. Kane and John N. Banks -- Choosing and characterizing antibodies / Anthony Forde and John Coley -- Labels and endpoints / Wajdi Abdul-Ahad and Martin Brett -- Solid-phase reagents / Robert S. Matson -- Standards and immunogens / M. Quasim Chaudry -- Optimization / Eric Ezan and Jacques Grassi -- Validation / Peter O\'Fegan -- Data processing / Barry Nix and David Wild -- Quality assurance / John Seth.

CIT Module BIOT 7002 - Core reading.

CIT Module BIOT 8004 - Core reading.

Table of contents provided by Syndetics

  • Analysis by specific binding
  • Raising antibodies
  • Choosing and characterizing antibodies
  • Labels and endpoints
  • Solid-phase reagents
  • Standard and immunogens
  • OptimizationValidation
  • Data processing
  • Quality assurance

Author notes provided by Syndetics

JimGoslingDepartment of BiochemistryNational University of Ireland.