MTU Library Catalogue

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Fundamental laboratory approaches for biochemistry and biotechnology : a text with experiments / Alexander J. Ninfa and David P. Ballou.

By: Ninfa, Alexander J.
Contributor(s): Ballou, David P.
Material type: materialTypeLabelBookPublisher: Hoboken, NJ : Wiley, 2004Description: xi, 372 p. : ill. ; 28 cm.ISBN: 1891786008 .Subject(s): Biochemistry -- Laboratory manuals | Biotechnology -- Laboratory manuals | Molecular biology -- Laboratory manualsDDC classification: 572
Contents:
Basic practices and techniques in the biochemistry laboratory -- Spectrophotometry -- Quantification of protein concentration -- Chromatography -- Gel electrophoresis of proteins -- Overview of protein purification -- Isolation and characterization of the enzyme alkaline phosphatase from escherichia coli -- Enzyme kinetics -- Enzymatic methods of analysis -- Ligand binding -- Recombinant DNA techniques -- Polymerase chain reaction (PCR) technology -- Using the personal computer and the internet for biochemical research.
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Bishopstown Library Lending 572 (Browse shelf(Opens below)) 1 Available 00079150
Total holds: 0

Enhanced descriptions from Syndetics:

An excellent biochemistry laboratory text for advanced undergraduate and first year graduate students in biochemistry and other life sciences, this text provides a logical framework for training students how to approach research problems and conduct and evaluate scientific research. Each chapter provides extensive background on the principles underlying methods used to research, followed by experiments designed to illustrate those principles.

Includes bibliographical references and index.

Basic practices and techniques in the biochemistry laboratory -- Spectrophotometry -- Quantification of protein concentration -- Chromatography -- Gel electrophoresis of proteins -- Overview of protein purification -- Isolation and characterization of the enzyme alkaline phosphatase from escherichia coli -- Enzyme kinetics -- Enzymatic methods of analysis -- Ligand binding -- Recombinant DNA techniques -- Polymerase chain reaction (PCR) technology -- Using the personal computer and the internet for biochemical research.

Table of contents provided by Syndetics

  • Preface.
  • Chapter 1 Basic Practices and Techniques in the Biochemistry Laboratory
  • Chapter 2 Spectrophotometry
  • Chapter 3 Quantification of Protein Concentration
  • Chapter 4 Chromatography
  • Chapter 5 Gel Electrophoresis of Proteins
  • Chapter 6 Overview of Protein Purification
  • Chapter 7 Isolation and Characterization of the Enzyme Alkaline Phosphatase From Escherichia Coli
  • Chapter 8 Enzyme Kinetics
  • Chapter 9 Enzymatic Methods of Analysis
  • Chapter 10 Ligand Binding
  • Chapter 11 Recombinant DNA Techniques
  • Chapter 12 Polymerase Chain Reaction (PCR) Technology
  • Chapter 13 Using the Personal Computer and the Internet for Biochemical Research
  • Appendix 1 Safety Checklist
  • Appendix 2 An Example of an MSDS
  • Index

Author notes provided by Syndetics

Alexander J. Ninfa is Associate Professor in the Department of Biological Chemistry at the University of Michigan and is internationally recognized for his work on the biochemical mechanisms of signal transduction and transcriptional regulation in bacteria.

David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.