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Principles of gene manipulation : an introduction to genetic engineering / R. W. Old and S. B. Primrose.

By: Old, R. W.
Contributor(s): Primrose, S. B.
Material type: materialTypeLabelBookSeries: Studies in microbiology.Publisher: Oxford : Blackwell Scientific, 1994Edition: 5th ed.Description: vi, 474 p. : ill. ; 25 cm.ISBN: 0632037121.Subject(s): Genetic engineeringDDC classification: 575.10724
Contents:
Gene manipulation: an all-embracing technique -- Basic techniques -- Cutting and joining DNA molecules -- Plasmids as cloning vehicles for use in E. coli --- Bacteriophage and cosmid vectors for E. coli -- Cloning strategies, gene libraries and cDNA cloning -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Analysing DNA sequences -- The polymerase chain reaction -- Changing genes: site-directed mutagenesis -- Cloning in bacteria other than E. coli -- Cloning in Saccharomyces cerevisiae and other microbial eukaryotes -- Gene transfer to plants -- Introducing genes into animal cells -- Transferring genes into animal oocytes, eggs, embryos and specific animal tissues -- The impact of recombinant DNA technology: the generation of novelty.
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Bishopstown Library Lending 575.10724 (Browse shelf(Opens below)) 1 Available 00009793
Total holds: 0

Enhanced descriptions from Syndetics:

As in past editions, the basic philosophy of this extremely popular text is to present the principles and techniques of gene manipulation in sufficient detail to enable the non-specialist to understand them. However, in the three years since the previous edition, molecular biology has seen a dramatic increase in available techniques and applications. Every chapter in this new edition has been extensively revised or rewritten to take these developments into account - including a vastly expanded section on the polymerase chain reaction and its manifold applications, a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics, and extended coverage of gene transfer techniques.

Includes bibliographical references (pages 412-464) and index.

Gene manipulation: an all-embracing technique -- Basic techniques -- Cutting and joining DNA molecules -- Plasmids as cloning vehicles for use in E. coli --- Bacteriophage and cosmid vectors for E. coli -- Cloning strategies, gene libraries and cDNA cloning -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Analysing DNA sequences -- The polymerase chain reaction -- Changing genes: site-directed mutagenesis -- Cloning in bacteria other than E. coli -- Cloning in Saccharomyces cerevisiae and other microbial eukaryotes -- Gene transfer to plants -- Introducing genes into animal cells -- Transferring genes into animal oocytes, eggs, embryos and specific animal tissues -- The impact of recombinant DNA technology: the generation of novelty.

Table of contents provided by Syndetics

  • Introduction to Gene Manipulation
  • Why do we clone genes?
  • How do we clone genes?- the basic technique
  • Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors
  • Phage and cosmid vectors
  • Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes
  • Analyzing DNA nucleotide sequences
  • Changing genes - macro- and micro-modifications
  • The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics
  • Cloning in Organisms other than E. coli
  • Cloning in bacteria other than E. coli
  • Cloning in yeast and other microbial eukaryotes
  • Gene transfer to plants
  • Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty