Enhanced descriptions from Syndetics:

Adding 13 examples to this new, third edition, Veronika Meyer now offers solutions for nearly 100 problems. All the examples are accompanied by a concise, instructive text and an informative figure, including essential fundamentals as well as such helpful strategies as equipment tests or quality assurance strategies. A practice-oriented aid to obtaining correct and reliable analytical results.

From the reviews of a previous edition:
'This is a giant of a little book on HPLC.'
(Analytical Chemistry)

Table of contents provided by Syndetics

• Preface (p. VII)
• Introduction (p. 1)
• Part I Fundamentals (p. 3)
• 1.1 Chromatography (p. 4)
• 1.2 Chromatographic Figures of Merit (p. 6)
• 1.3 The Resolution of Two Peaks (p. 8)
• 1.4 Reduced Parameters (p. 10)
• 1.5 The Van Deemter Curve (p. 12)
• 1.6 Peak Capacity and Number of Possible Peaks (p. 14)
• 1.7 Statistical Resolution Probability: Simulation (p. 16)
• 1.8 Statistical Resolution Probability: Example (p. 18)
• 1.9 Precision and Accuracy of an Analytical Result (p. 20)
• 1.10 Standard Deviation (p. 22)
• 1.11 Uncertainty Propagation (p. 24)
• 1.12 Reproducibility in Trace Analysis (p. 26)
• 1.13 Ruggedness (p. 28)
• 1.14 Calibration Curves (p. 30)
• 1.15 The HPLC Instrument (p. 32)
• 1.16 The Detector Response Curve (p. 34)
• 1.17 Noise (p. 36)
• 1.18 Causes and Effects Presented as an Ishikawa Diagram (p. 38)
• 1.19 The Possible and the Impossible (p. 40)
• Part II Pitfalls and Sources of Error (p. 43)
• 2.1 Mixing of the Mobile Phase (p. 44)
• 2.2 Mobile Phase pH (p. 46)
• 2.3 Adjustment of Mobile Phase pH (p. 48)
• 2.4 Inadequate Purity of a Mobile Phase Solvent (p. 50)
• 2.5 Inadequate Purity of a Mobile Phase Reagent (p. 52)
• 2.6 System Peaks and Quantitative Analysis (p. 54)
• 2.7 Sample Preparation with Solid Phase Extraction (p. 56)
• 2.8 Poor Choice of Sample Solvent: Peak Distortion (p. 58)
• 2.9 Poor Choice of Sample Solvent: Tailing (p. 60)
• 2.10 Sample Solvent and Calibration Curve (p. 62)
• 2.11 Impurities in the Sample (p. 64)
• 2.12 Formation of a By-Product in the Sample Solution (p. 66)
• 2.13 Decomposition by the Sample Vial (p. 68)
• 2.14 Artifact Peaks from the Vial Septum (p. 70)
• 2.15 Formation of an Associate in the Sample Solution (p. 72)
• 2.16 Precision and Accuracy with Loop Injection (p. 74)
• 2.17 Injection Technique (p. 76)
• 2.18 Injection of Air (p. 78)
• 2.19 Sample Adsorption in the Loop (p. 80)
• 2.20 Extra-Column Volumes (p. 82)
• 2.21 Dwell Volume (p. 84)
• 2.22 Elution at t[subscript 0] (p. 86)
• 2.23 Classification of C[subscript 18] Reversed Phases (p. 88)
• 2.24 Different Selectivity of C[subscript 18] Reversed Phases (p. 90)
• 2.25 Different Batches of Stationary Phase (p. 92)
• 2.26 Chemical Reaction within the Column (p. 94)
• 2.27 Recovery and Peak Shape Problems with Proteins (p. 96)
• 2.28 Double Peaks from Stable Conformers (p. 98)
• 2.29 Influence of Temperature on the Separation (p. 100)
• 2.30 Thermal Non-Equilibrium within the Column (p. 102)
• 2.31 Influence of the Volume Flow Rate on the Separation (p. 104)
• 2.32 Influence of Run Time and Volume Flow Rate on Gradient Separations (p. 106)
• 2.33 UV Spectra and Quantitative Analysis (p. 108)
• 2.34 UV Detection Wavelength (p. 110)
• 2.35 Fluorescence Quenching by Air (p. 112)
• 2.36 Detector Overload (p. 114)
• 2.37 Influence of the Retention Factor on Peak Height (p. 116)
• 2.38 Influence of the Volume Flow Rate on Peak Area (p. 118)
• 2.39 Leaks in the HPLC Instrument (p. 120)
• 2.40 Impairment of Precision as a Result of Noise (p. 122)
• 2.41 Determination of Peak Area and Height at High Noise (p. 124)
• 2.42 Peak Height Ratios (p. 126)
• 2.43 Incompletely Resolved Peaks (p. 128)
• 2.44 Area Rules for Incompletely Resolved Peaks (p. 130)
• 2.45 Areas for a 1 : 10 Peak Pair (p. 132)
• 2.46 Heights for a 1 : 10 Peak Pair (p. 134)
• 2.47 Quantitative Analysis of a Small Peak (p. 136)
• 2.48 Incompletely Resolved Peaks with Tailing (p. 138)
• 2.49 Integration Threshold and Number of Detected Peaks (p. 140)
• 2.50 Detector Time Constant and Peak Shape (p. 142)
• 2.51 Quantitative Analysis in the 99 % Range (p. 144)
• 2.52 Correlation Coefficient of Calibration Curves (p. 146)
• Part III Useful Strategies (p. 149)
• 3.1 Column Tests (p. 150)
• 3.2 Apparatus Tests (p. 152)
• 3.3 Wavelength Accuracy of the UV Detector (p. 154)
• 3.4 Internal Standards (p. 156)
• 3.5 A Linearity Test (p. 158)
• 3.6 Rules for Accurate Quantitative Peak Size Determination (p. 160)
• 3.7 High-Low Chromatography (p. 162)
• 3.8 Control Charts (p. 164)
• 3.9 Verification of the Analytical Result by Use of a Second Method (p. 166)
• 3.10 Description of Ruggedness (p. 168)
• 3.11 Rules for Passing On an HPLC Method (p. 170)
• 3.12 Quality Assurance in the Laboratory (p. 172)
• 3.13 Standard Operating Procedures (p. 174)
• 3.14 Method Validation (p. 176)
• 3.15 Some Elements of Validation (p. 178)
• 3.16 A Validation Example (p. 180)
• 3.17 Measurement Uncertainty (p. 182)
• 3.18 Formal Quality Assurance Systems (p. 184)
• Index (p. 187)

Author notes provided by Syndetics