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Bacteriophages methods and protocols. Vol. 1, Isolation, characterization, and interactions / edited by Martha R. J. Clokie and Andrew M. Kropinski.

Contributor(s): Clokie, Martha R. J | Kropinski, Andrew M. (Andrew Maitland Boleslaw), 1943-.
Material type: materialTypeLabelBookSeries: Methods in molecular biology ; 501.Publisher: New York : Springer, 2009Description: xxii, 307 p. : ill. ; 27 cm. + hbk.ISBN: 9781588296825 .Subject(s): BacteriophagesDDC classification: 579.26
Contents:
Section 1 Bacteriophage isolation: Methods for the isolation of viruses from environmental samples / K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench and Danielle M. Winget -- Bacteriophage enrichment from water and soil / Rohan Van Twest and Andrew M. Kropinski -- Isolation of phage via induction of lysogens / Raul R. Raya and Elvira M. Herbert -- Isolation of cyanophages from aquatic environments / Andrew D. Millard -- Isolation of viruses from high temperature environments / Jennifer Fulton, Trevor Douglas and Mark Young -- Isolation of novel large and aggregating bacteriophages / Philip Serwer, Shirley J. Hayes, Julie A. Thomas, Borries Demeler and Stephen C. Hardies -- Section 2 Bacteriophage characterization: Enumeration of bacteriophages by double agar overlay plaque assay / Andrew M. Kropinski, Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr and Roger P. Johnson -- Enumeration of bacteriophages by the direct plating plaque assay / Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr and Roger P. Johnson -- Enumeration of bacteriophages using the small drop plaque assay system / Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr and Roger P. Johnson -- Determination of virus abundance by epifluorescence microscopy / Alice C. Ortmann and Curtis A. Suttle -- Enumeration of bacteriophages using flow cytometry / Corina P. D. Brussaard -- Basic phage electron microscopy / Hans-W. Ackermann -- Phage classification and characterization / Hans-W. Ackermann -- Phage host range and efficiency of plating / Elizabeth Kutter -- Measurement of the rate of attachment of bacteriophage to cells / Andrew M. Kropinski -- Measurement of the bacteriophage inactivation kinetics with purified receptors / Andrew M. Kropinski -- Bacteriophage plagues: theory and analysis / Stephen T. Abedon and John Yin -- Practical methods for determining phage growth parameters / Paul Hyman and Stephen T. Abedon -- Phage production and maintenance of stocks, including expected stock lifetimes / Louis-Charles Fortier and Sylvain Moineau -- Section 3 Bacteriophage-host interaction: Construction of phage mutants / Robert Villafane -- Modifying bacteriophage with recombineering / Lynn C. Thomason, Amos B. Oppenheim and Donald L. Court -- Identification and isolation of lysogens with induced prophage / Jonathan Livny, Christopher N. LaRock and David I. Friedman -- Generalized transduction / Anne Thierauf, Gerardo Perez and Stanley Malow -- Preparation and characterization of anti-phage serum / Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco and Roger P. Johnson -- Generalized transduction by lytic bacteriophages / Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, Andrew M. Kropinski and Roger P. Johnson.
Holdings
Item type Current library Call number Copy number Status Barcode
General lending MTU Bishopstown Library Lending 579.26 (Browse shelf(Opens below)) 1 Available 00183821
Total holds: 0

Enhanced descriptions from Syndetics:

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

Includes bibliographical references and index.

Section 1 Bacteriophage isolation: Methods for the isolation of viruses from environmental samples / K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench and Danielle M. Winget -- Bacteriophage enrichment from water and soil / Rohan Van Twest and Andrew M. Kropinski -- Isolation of phage via induction of lysogens / Raul R. Raya and Elvira M. Herbert -- Isolation of cyanophages from aquatic environments / Andrew D. Millard -- Isolation of viruses from high temperature environments / Jennifer Fulton, Trevor Douglas and Mark Young -- Isolation of novel large and aggregating bacteriophages / Philip Serwer, Shirley J. Hayes, Julie A. Thomas, Borries Demeler and Stephen C. Hardies -- Section 2 Bacteriophage characterization: Enumeration of bacteriophages by double agar overlay plaque assay / Andrew M. Kropinski, Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr and Roger P. Johnson -- Enumeration of bacteriophages by the direct plating plaque assay / Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr and Roger P. Johnson -- Enumeration of bacteriophages using the small drop plaque assay system / Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr and Roger P. Johnson -- Determination of virus abundance by epifluorescence microscopy / Alice C. Ortmann and Curtis A. Suttle -- Enumeration of bacteriophages using flow cytometry / Corina P. D. Brussaard -- Basic phage electron microscopy / Hans-W. Ackermann -- Phage classification and characterization / Hans-W. Ackermann -- Phage host range and efficiency of plating / Elizabeth Kutter -- Measurement of the rate of attachment of bacteriophage to cells / Andrew M. Kropinski -- Measurement of the bacteriophage inactivation kinetics with purified receptors / Andrew M. Kropinski -- Bacteriophage plagues: theory and analysis / Stephen T. Abedon and John Yin -- Practical methods for determining phage growth parameters / Paul Hyman and Stephen T. Abedon -- Phage production and maintenance of stocks, including expected stock lifetimes / Louis-Charles Fortier and Sylvain Moineau -- Section 3 Bacteriophage-host interaction: Construction of phage mutants / Robert Villafane -- Modifying bacteriophage with recombineering / Lynn C. Thomason, Amos B. Oppenheim and Donald L. Court -- Identification and isolation of lysogens with induced prophage / Jonathan Livny, Christopher N. LaRock and David I. Friedman -- Generalized transduction / Anne Thierauf, Gerardo Perez and Stanley Malow -- Preparation and characterization of anti-phage serum / Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco and Roger P. Johnson -- Generalized transduction by lytic bacteriophages / Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, Andrew M. Kropinski and Roger P. Johnson.