Search for 'su:"Paratuberculosis"'

Isolation and molecular characterization of Mycobacterium avium subsp.paratuberculosis in Ireland /

]]> By Douarre, P. E..
Cork : Cork Institute of Technology, 2010 .
xv, 208 p. : 30 cm. +.

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Identification and molecular characterisation of mycobacteriophages and their lysis proteins /

]]> By Henry, Marine.
Cork : Cork Institute of Technology, 2010 .
xvi, 270 p. : 30 cm. +.

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Development of a recombinant Lactobacillus salivarius for prophylactic applications against Mycobacterium avium subspecies paratuberculosis and Johne's disease /

]]> By Johnson, Christopher,.
Cork : Cork Institute of Technology, 2013 .
188 pages : 30 cm..

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Exploiting bacteriophages and associated peptidoglycan hydrolases with potential for biocontrol in food related applications /

]]> By Endersen, Lorraine,.
.
v, 477 pages : 30 cm.

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An investigation of the use of natural agents to control mycobacteria /

]]> By Lesniak, Olga,.
.
103 pages : 30 cm.

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A diagnostic and control programme for Johne's Disease on Irish dairy farms /

]]> By Kennedy, Aideen.
.
229 pages : , Johne's disease (JD) is an enteritis of ruminants with potential zoonotic implications. JD diagnosis ad control present difficulties give JDs prolonged subclinical nature. In order to improve JD control in Ireland, PhD aims included conducting a national survey documenting high-risk husbandry practices employed on Irish farms, thereby targeting areas for improved management. Furthermore as JD has been associated with on-farm economic losses internationally, this research programmed aimed to analyse production losses associated with testing JD ELISA positive on Irish dairy farms. A longitudinal study was also conducted to assess if successful JD control can be achieved using gold-standard protocols. Due to the prolonged nature of the disease, diagnosis of MAP is notoriously difficult. None of the available tests for MAP report perfect test sensitivity or specificity. A further complicating issue in Ireland, is the high level of bovine tuberculosis (bTB) testing that is conducted. Multiple investigations relating to bTB-testing and its relationship with MAP diagnostics were conducted. Across all investigations, 312 farms were recruited, samples were collected from 4500 cows, and 10000 test results were generated (ELISA, PCR, faecal culture). Irish farms engaged in high-risk management practices facilitating JD transmission, however, no significant associations were identified between JD ELISA positivity and production parameters. On-farm sero-prevalence was decreased by implementation of gold standard protocols. Eradication was not achieved, however, highlighting the long-term commitment required for effective JD control. Annual bTB testing was associated with an increased prevalence of JD ELISA positive results and indicates that sampling for the purposes of JD surveillance should be avoided for 71 days post- TB test administration. Antibody responses and interferon-y production were significantly increased post-b TB testing. As cell-mediated immunity is particularly important in the control of JD systemically, bTB testing may be contributing to the low levels of clinical JD in Ireland and warrants further investigation. This research programme has greatly contributed to our knowledge of JD in Ireland and improves understanding of practical measures necessary to interpret ELISA diagnostics and aid JD control - (Abstract) | This Ph.D is a joint research project between Teagasc Moorepark and Cork Institute of Technology. 30 cm.

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The development of new molecular tools to detect Mycobacterium avium subspecies paratuberculosis from a bovine faecel environment by real-time PCR /

]]> By Kruijf, Marcel de.
.
201 pages : , The slow-growing intracellular pathogen Mycobacterium Avium subspecies Paratuberculosos (MAP) causes Johne's Disease in domesticated and wild ruminants. Johne's disease inflicts severe annual economic losses to the dairy industry worldwide. This thesis is divided into five chapters that consist of a literature review chapter and four experimental chapters focusing on MAP strain genotyping and the development of novel molecular MAP detection tools using real-time PCR. Chapter 1 describes published information regarding MAP characteristics, Johne's disease, MAP genomics, available MAP detection methods, MAP epidemiology and genotyping. To efficiently control and monitor MAP infection, molecular typing of MAP strains provides extensive knowledge regarding the source and genetic diversity of MAP within a geographic location. Knowledge regarding MAP genotypes within Ireland is limited and therefore, a large collection of MAP isolates from 53 herds across the Republic of Ireland were genotyped using the MIRU-VNTR technique and described in chapter 2. Rapid detection of MAP from faeces using real-time PCR is crucial to halt the infectious spread within herds. MAP- specific multi-copy elements are attractive and sensitive molecular targets for real-time PCR assays to detect MAP from faeces. The multi-copy IS_MAP04 has never previously been evaluated as a potential novel target in MAP diagnostics. This evaluation is described in chapter 3. A further MAP-specific molecular target has been investigated and described in chapter 4. The truncation present in the mycobactin cluster within MAP was used as a novel MAP-specific target in real-time PCR assays to detect and conform MAP from faeces. The elimination of false-negative detection results is another crucial aspect in molecular MAP diagnostics. Chapter 5 describes the development of two novel MAP DNA extraction and real-time PCR internal controls using Mycobacterium smegmatis as a model mycobacterial strain. These developed internal controls could aide in the elimination of false-negative MAP detection results from faeces - (Abstract) 30 cm.

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